How to Make an Orthotopic Model of Uterine Cancer in mouse?
(Protocol for an orthotopic uterine cancer model in mouse)
Materials:
1. Surgical scissors, forceps, and scalpel.
2. 70% ethanol wipes and Betadine swabs.
3. An autoclip wound applier and stainless-steel autoclips.
4. Q-tips
5. 30-gauge needles
6. A 1-cc syringe
7. Tumor cells
Anesthetics:
Methoxyflurane
Methods:
The mouse uterus has a pair of relative long horns lying in the dorsal abdominal cavity. The body and short cervix are located between the urinary bladder and the rectum.
1. Anesthetize a nude mouse with methoxyflurane inhalation.
2. Place the mouse on its back.
3. Scrub the lower abdomen wall with Betadine followed by 70% ethanol.
4. Incise the skin and peritoneum along the lower midline for about1 cm. Expose and stabilize the uterus gently.
5. Insert a 30-gauge needle attached to a 1-cc syringe obliquely into the uterine wall at the level of the cervix (the cervix is the most common site of cancer development).
6. Inject 50 µl of the material slowly. A visible pale wheal indicates a successful injection.
7. Retract the needle.
8. Place a Q-tip over the injection site for about 30 second to prevent bleeding and spillage of material.
9. Close the abdominal wall with wound autoclip in one layer
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