How to make a subcutaneous (SC or s.c.) cancer model in mouse?
(Protocol for a subcutaneous cancer model in mouse)
Materials:
A 27-gauge or smaller needle
A 1-cc syringe
Tumor cells
Methods:
Using the loose skin in the right flank as the injection site so that the measurement of the subsequent growing tumor will be easier. Restrain the mouse properly, after disinfecting the skin with 70% ethanol wipes insert a 27-gauge or smaller needle firmly through the skin and further advance a few millimeters, lift up the needle tip a bit to make sure that it is not in muscle, inject the tumor cells in 0.1 ml. Retract the needle, Place a Q-tip over the injection site for 30 seconds to prevent bleeding and spillage of tumor cells.
To learn more proven tips about mouse orthotopic model of subcutaneous cancer, please see Mouse Experiment Tips. If you want to see the live demos of this specific technique by mouse work experts, please visit Video Training Courses.