Mouse Cancer Model

1. Surgical scissors, forceps, and scalpel.
2. 70% ethanol wipes and Betadine swabs.
3. An autoclip wound applier and stainless-steel autoclips.
4. Q-tips
5. 27- or 30-gauge needles
6. A 1-cc syringe
7. Tumor cells
Anesthetics:

Methoxyflurane

Methods:

The mouse prostate consists of distinctive anterior, dorsal and ventral lobes, lying in the caudal part of abdominal cavity. The anterior lobes are attached to the seminal vesicles. The dorsal lobes surround the urethra, the ventral lobe sits between the urethra and the urinary bladder. The dorsal lobes are commonly used to inject prostate cancer cells.

1. Anesthetize a nude mouse with methoxyflurane inhalation.
2. Place the mouse on its back.
3. Scrub the lower abdomen wall with Betadine followed by 70% ethanol.
4. Incise the skin and peritoneum along the lower midline for about 1 cm. Exteriorize the prostate dorsal lobes and stabilize them gently.
5. Insert a 30-gauge needle attached to a 1-cc syringe into the right dorsal lobe of the prostate.
6. Inject 50 µl the material slowly. A well-localized bleb indicates a successful injection.
7. Retract the needle.
8. Place a Q-tip over the injection site for about 1 min to prevent bleeding and spillage of material.
9. Return the prostate to the peritoneum.
10. Close the abdominal wall with wound autoclip in one layer.