How to Make a Liver/Hepatic Metastatic Cancer Model by Intracecal Injection in mouse?
(Protocol for a Liver/Hepatic Metastatic Cancer Model by Intracecal Injection in mouse)
Materials:
1. Surgical scissors, forceps, and scalpel.
2. 70% ethanol wipes and Betadine swabs.
3. An autoclip wound applier and stainless-steel autoclips.
4. Q-tips
5. 30-gauge needles
6. A 1-cc syringe
7. Tumor cells
Anesthetics:
Methoxyflurane
Methods:
The mouse cecum is a blind pouch-like fragment of the colon into which the ileum opens, located in the left ventrocaudal abdominal cavity, easy to be located.
1. Anesthetize a nude mouse with methoxyflurane inhalation.
2. Place the mouse on its back.
3. Scrub the the lower abdomen with Betadine followed by alcohol.
4. Incise the skin and peritoneum through the lower midline for about 1 cm.
5. Exteriorize the cecum.
6. Insert a 30-gauge needle on a 1-cc syringe obliquely into the cecal wall.
7. Inject 50 µL tumor cell suspension slowly. A well-localized fluid bleb indicates a successful injection.
8. Retract the needle.
9. Place a Q-tip over the injection site for 30 seconds to prevent bleeding and spillage of the material.
10. Return the cecum to the peritoneum.
11. Close the wound with wound clip in one layer.
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