How to Make an Orthotopic Model of Gastric Cancer in mouse?
(Protocol for an orthotopic gastric cancer model in mouse)
Materials:
1. Surgical scissors, forceps, and scalpel.
2. 70% ethanol wipes and Betadine swabs.
3. An autoclip wound applier and stainless-steel autoclips.
4. Q-tips
5. 27- or 30-gauge needles
6. A 1-cc syringe
7. Tumor cells
Anesthetics:
Methoxyflurane
Methods:
The mouse stomach lies in the left cranial part of the abdominal cavity, left to and partially covered by the left hepatic lobe.
1. Anesthetize a nude mouse with methoxyflurane inhalation.
2. Place the mouse on its back.
3. Scrub the abdomen wall with betadine followed by alcohol.
4. Incise the skin and peritoneum along the upper midline for about 2 cm. Exteriorize the stomach and stabilize it gently.
5. Insert a 27- or 30-gauge needle on a 1-cc syringe into the wall of stomach. Inject 50 µl of the material slowly.
6. A visible bulla indicates a successful injection.
7. Retract the needle.
8. Place a Q-tip over the injection site for about 1 min to prevent bleeding and spillage of material.
9. Return the stomach to the peritoneum.
10. Close the abdominal wall with wound autoclip in one layer.
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