Mouse Cancer Model

(Protocol for an orthotopic bone cancer model in mouse)


Materials:

1. 70% ethanol wipes and Betadine swabs.
2. An autoclip wound applier and stainless-steel autoclips.
3. Q-tips
4. 27 gauge needle
5. A 1-cc syringe
6. Tumor cells

Anesthetics:

Numbutal (pentobarbital sodium)

Methods:

1. Anesthetize a mouse by intraperitoneal injection of Nembutal.
2. Disinfect the skin over the knee joint of left hind limb with betadine and 70% ethanol.
3. Bend the knee to best expose proximal end of the tibia.
4. Insert 27-Gauge needle through the skin into the tibia immediately proximal to the tuberositas tibiae.
5. Drill the needle to penetrate the cortical bone and continue to advance to the cancellous bone of the shaft.
6. Inject 20 µl of the cell suspension slowly into the bone.
7. Retract the needle,
8. Place a Q-tip over the injection site for about 1 min to prevent bleeding and spillage of material.

 

To learn more proven tips about mouse orthotopic model of bone cancer, please see Mouse Experiment Tips.

 

If you want to see the live demos of this specific technique by mouse work experts, please visit Video Training Courses.