How to do Intraperitoneal (IP) injection?
Materials:
1. 70% ethanol wipes.
2. 25 –, 27 – or 30 – gauge needles
3. A 1–cc syringe
Methods:
Intraperitoneal injection has been widely used for mouse anesthesia or drug administration. A mouse is manually restrained with the right hind limb immobilized and the head and body tilted downward. The right lower abdominal wall is disinfected with 70% ethanol. A 25 – gauge or smaller needle is quickly inserted into the peritoneal cavity in the caudal right abdominal quadrant through the skin and the abdominal wall, thereby avoiding injection into the cecum or the stomach on the left side. The needle tip is then lifted slightly and the material is delivered.
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